Movies and Microscopy


 

 

Localization of E66 and 33 E66 EGFP During Infection (by Shawn Williamson)
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SF9 Cells were infected with a recombinant virus that expresses the putative N-terminal signature motif of ODV-E66 fused to EGFP under the polyhedrin promoter (pol). Infected cells were collected at 48 hours post infection and imaged confocally using a Zeiss Axiovert 135 with the CARV confocal module. This movie is a confocal stack of the entire infected cell with each frame being representative of a 0.75 micron slice. The red label is "native" ODV-E66, the green label is the pol expressed fusion product. ODV-E66 localizes primarily to virally induced intranuclear microvesicles during infection with this virus. Co-localization of the fusion protein with E66 is indicated by a yellow label.

 

The Infection and Maturation of the Baculovirus (designed by Jared Burks)
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