Know:
1. why addition of intermediates of the TCA cycle consume more
oxygen than required for their complete oxidation.
2. the coenzyme responsible for decarboxylation of alpha-ketoacids
3. mechanism of pyruvate DH complex, citrate synthase, aconitase,
isocitrate DH, alphKGDH complex, succinyl-CoA synthetase, succinate
DH, fumarase. Malate DH
4. for the alphaketoacid DH complexes, free energy conserved in
formation od NADH2 and thioester
5. which enzyme catalyzes substrate-level phosphorylation
6. TCA cycle the combination of two ancient pathways
7. why acetate C are not oxidized to CO2 in the first turn of the cycle
8. regulation of TCA cycle by NAD/NADH2 ratio at dehydrogenases;
regulation of isocitrate DH; regulation of pyruvate DH complex