Questions 1-6 2 pts each, questions 7-30 3pts each: total 84pts
Match the speaker with the topic.
| 1. | Reinhart | A. | phytochemicals |
| 2. | Kladde | B. | rice genetic engineering |
| 3. | Harris | C. | crystallography and drug design |
| 4. | Park | A. | AIDS and HIV |
| 5. | Sacchettini | B. | allosteric models |
| 6. | LiWang | C. | chromosome structure |
7. Allosteric regulators change the catalytic activity of an enzyme by binding to:
A. the active site
B. between subunits
C. the hydrophyllic sites
D. the hydrophobic sites
E. a site other than the active site
8. If B is the curve in the absence of a allosteric regulators, allosteric inhibition is shown by which curve?
9. Properties of the allosteric enzyme phosphofructokinase are:
A. an important regulatory enzyme in lipid (fat) metabolism
B. a heterotetramer with two a and two b subunits
C. catalyzes Fru6P + MgATP ı Fru16BP + MgADP
D. allosterically activated by phosphoenolpyruvate, PEP
E. allosterically inhibited by MgADP
10. Two models of allosteric regulation are concerted and sequential. Experiments on phosphofructokinase showed that allosteric regulation by phosphoenolpyruvate is:
A. concerted
B. sequential
C. neither sequential or concerted
D. concerted at 3 sites and sequential at 1
E. sequential at 3 sites and concerted at 1
11. Site directed mutagenesis is a technique for:
A. specifically changing one amino acid for another in a gene
B. detecting splice sites in mRNA
C. detecting histone binding sites on DNA
D. increasing rates of protein crystallization
E. increasing rates of DNA methylation
12. DNA contains:
A. deoxyribose
B. two antiparallel chains
C. A+G = C+T
D. phosphodiester backbone outside
E. all the above
13. In a nucleosome:
A. positively charged histone proteins bind to negatively charged DNA
B. negatively charged histone proteins bind to positively charged DNA
C. histones unwind DNA for replication and transcription
D. histones stabilize single stranded DNA during replication and transcription
E. histones methylate DNA
14. Acetylation of histones by the enzyme histone acetyltransferase:
A. changes the 10nm chromatin fiber into a 30nm chromatin fiber
B. unwinds the double helix of DNA
C. causes histones to methylate specific C residues in DNA
D. changes the net charge on histones from + to neutral
E. changes the net charge on histones from to +
15. Yeast interprets environmental signals such as extracellular [phosphate]. When [phosphate] is low:
A. PHO system is off.
B. Pho4 is at [high] in the nucleus
C. Pho4 is at [high] in the cytoplasm
D. Pho4 is phosphorylated
E. Pho4 methylates the PHO system
16. Limonoids:
A. have a single ring structure
B. have tasteless aglycans
C. have bitter tasting glucosides
D. are antioxidants
E. all the above
17. Limonoid glucosides:
A. quench free radicals
B. induce apoptosis
C. trigger caspase activity
D. are toxic to neuroblastoma cells
E. all the above
18. Examples of antioxidants are:
A. vitamin C
B. vitamin E
C. superoxide dismutase
D. catalase
E. all the above.
19. Golden Rice is a notable biotechnology achievement. Golden Rice was engineered with genes from bacteria and plants to make:
A. vitamin A
B. vitamin C
C. vitamin E
D. beta-carotene
E. anticancer limonoids
20. "Green" organizations such as Greenpeace are against Golden Rice and GM crops because:
A. they are "Luddites" and anti-technology
B. genetic novelty introduces new allergens
C. transgenes may escape to related weeds
D. GM crops displace native species and reduce genetic diversity
E. all the above
21. The critical gene affecting the storage and cooking properties of brown rice was:
A. phosphofructokinase
B. lipid metabolism
C. amylose (starch) synthesis
D. amylose (starch) breakdown
E. limonoid synthesis
22. The GıT mutation found in the 2+ rice variety:
A. is temperature sensitive
B. is at an exon-intron junction
C. introduces an alternative splice-site
D. introduces different cooking and storage properties
E. all the above
23. Goal/s of structural genomics is/are:
A. define the types of tertiary structure folds
B. deduce function from structure
C. understand the protein folding problem
D. understand protein-protein interactions
E. all the above
24. The technique for determining the position of each atom in a protein is:
A. nuclear magnetic resonance (NMR)
B. electron microscopy
C. X-ray crystallography
D. computer graphics
E. all the above
25. The secondary structure/s found in proteins is/are:
A. alpha helix
B. beta sheet
C. coils
D. A and B
E. A, B and C
26. New drug development strategies for TB target:
A. phosphofructokinase
B. isocitrate lyase and malate synthase (not found in humans)
C. viral reverse transcriptase
D. viral protease
E. limonoid biosynthesis
27. HIV gains entry to T4 lymphocytes by binding to cell surface:
A. CD4 and CCR5 proteins
B. gp120 protein
C. gp41 protein
D. MIP-1a protein
E. T20 protein
28. Nucleoside analogs such as 3-azidothymidine inhibit:
A. reverse transcriptase
B. RNA polymerase
C. DNA polymerase
D. A and B
E. A, B and C
29. The HIV protease is essential for the life cycle of HIV because it hydrolyzes:
A. HIV coat protein
B. polyprotein product of HIV mRNA
C. cell surface CD4 and CCR5
D. reverse transcriptase
E. integrase
30. Strategy/ies for preventing HIV infection and AIDS is/are inhibition of:
A. cell surface binding
B. reverse transcriptase
C. integrase
D. protease
E. all the above
Answers:
1 B 2C 3A 4B 5C 6A 7E 8C 9C 10C 11A 12E 13A 14D 15B 16D 17E 18E 19A 20E 21C 22E 23E 24C 25E 26B 27A 28D 29B 30A