For questions #1-6, match the Lecturer to the topic.
| 1. Harris | A. Bioinformatics |
| 2. Guarino | B. Biotechnology in rice |
| 3. Park | C. Allosteric models |
| 4. Peterson | A. Oxidants and anti-oxidants |
| 5. Reinhart | B. SARS anti-virals |
| 6. Tsai | C. Transcriptional fidelity |
7. What happens when RNA polymerase II inserts the incorrect nucleotide?
A. transcription proceeds normally because RNA polymerase II has no proof reading capability
B. addition of the next nucleotide is increased by 100-times
C. the transcription bubble moves forward zipping up the DNA-RNA hybrid
D. a phosphodiestrase activity hydrolyzes the incorrect base pairing
E. the transcription bubble moves backwards unzipping the DNA-DNA hybrid
8. The molecular basis for RNA polymerase proof reading is the:
A. Watson-Crick base pairing in the DNA
B. insertion of canavanine in place of arginine
C. incorrect Watson-Crick base pairing in the DNA-RNA hybrid
D. competitive inhibition of the phosphodiesterase activity by the incorrect base
E. competitive inhibition of the polymerase activity by the incorrect base
9. Mutation of a normal codon to a stop codon in mRNA results in:
A. premature chain termination (smaller protein)
B. a single amino acid deletion in the protein
C. temperature-sensitive alternate splicing (protein with different properties)
D. insertion of canavanine in place of arginine
E. RNA polymerase II is arrested, backs up, and removes the incorrect base pair
10. The genome of SARS, a coronavirus, is a:
A. ssDNA
B. dsDNA
C. ssRNA
D. dsRNA
E. RNA-DNA hybrid
11. Subgenomic mRNAs of SARS all contain:
A. a protease for processing of the polyprotein product
B. an exonuclease for processing of the polycistronic mRNA
C. RNA polymerase specific for transcription from the 3'-end of the polycistronic mRNA
D. a leader sequence derived from the 5'-end of the polycistronic RNA
E. all the above
12. Nsp15 is a nuclease that hydrolyzes RNA on the 3'-side of RNA. The product using 5'-UGGGAGUAUA-3' is:
A. 5'-UGGGAGUAU-3'
B. 5'-UGGGAGU-3'
C. 5'-UGGGAG
D. 5'-GGGAG-3'
E. 5'-GGGAGU-3'
13. Which vitamin or vitamins are found in the outer layer of brown rice? Vitamin:
a. A
b. B1
c. C
d. D
e. E
Choose the correct answer.
A. a
B. a, b, c
C. b, c, d
D. c, d, e
E. a, b, e.
14. Which gene was found to be important for the cooking, storage and palatability of brown rice?
A. soluble starch synthase
B. granule bound starch synthase
C. polyprotein protease
D. phosphofructokinase
E. catalase
15. The product of granule bound starch synthase is:
A. sucrose
B. amylopectin
C. amylase
D. starch
E. branched amylase
16. In this enzyme kinetic plot of reaction velocity versus [substrate], if curve B is the plot in the absence of allosteric inhibitors or activators, the curves for the allosteric inhibitor and activator, respectively, are shown by:
A. A and C
B. C and A
C. none of the above
17. In allosteric homotetramers such as phosphofructokinase, binding at an allosteric site on one subunit is transmitted to an active site on another subunit by conformational changes involving:
A. H-bonds
B. charge-charge interactions
C. dipole interactions
D. the hydrophobic effect
E. all the above
18. The concerted and sequential models for allosteric proteins both postulate that substrate binds more readily to the:
A. tense (T) conformation
B. relaxed (R) conformation
C. tetramer conformation
D. the 3-1 conformation
E. the X-site on one subunit
19. What is the minimum number of active and allosteric binding sites on an allosteric protein?
A. one half of each
B. one of each
C. two of each
D. four of each
E. eight of each
20. The term kinase means that an enzyme (like phosphofructokinase):
A. uses fructose as a substrate
B. is inhibited by phosphoenolpyruvate (PEP)
C. uses ATP as a substrate
D. transfers phosphate from ATP to the substrate
E. transfers phosphate from PEP to ADP
21. The most dangerous free radical is:
A. H
B. OH
C. O2
D. agent orange
E. Fe(II)
22. Which enzyme is an anti-oxidant?
A. soluble starch synthase
B. granule bound starch synthase
C. polyprotein protease
D. phosphofructokinase
E. catalase
23. Which vitamins are anti-oxidants? Vitamin:
a. A
b. B1
c. C
d. D
e. E
Choose the correct answer.
A. a, b
B. b, c
C. c, e
D. a, b, d
E. a, b, e.
24. The two types of secondary structure in proteins are:
A. alpha helix and beta sheet
B. beta sheet and beta-turns
C. alpha helix and double helix
D. double helix and beta turns
E. alpha sheet and beta helix
25. The quaternary structure of proteins is held together by:
A. H-bonds
B. charge-charge interactions
C. dipole interactions
D. the hydrophobic effect
E. all the above
26. Explain why we need an arsenal of different anti-oxidants.
27. What are the strategies for targeting viral infections such as SARS or HIV?
28. Explain the problem of predicting the secondary and tertiary structures of proteins.
29. Why is rice a good choice for genetic manipulation?